Journal: Current Genomics

Article Title: Increasing the Coding Potential of Genomes Through Alternative Splicing: The Case of PARK2 Gene

doi: 10.2174/1389202915666140426003342

Figure Lengend Snippet: List of commercially available antibodies against human, rat and mouse PARK2.

Article Snippet: PAB0714 , Abnova , , , 305-323 , H1-H6, H8, H11, H17, H20, H21, R1, R2, R4-R10, R13-R15, R19.

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Changes in the Expression of TBP-2 in Response to Histone Deacetylase Inhibitor Treatment in Human Endometrial Cells

doi: 10.3390/ijms22031427

Figure Lengend Snippet: TRX and TBP-2 expression in endometrial cells, before and after treating with SAHA. ( A ) Relative mRNA levels of TRX, TBP-2 was presented according to the treatment doses. There was no difference in the mRNA level in Ishikawa cells, but there was a significant increase in HESCs up to 5 μM of SAHA treatment. ( B ) Representative immunoblots revealed increased TRX and TBP-2 in eutopic and ectopic HESCs, by Western blot; β-actin was used as an internal control.

Article Snippet: After blocking with BSA at room temperature for 1 h, the membranes were incubated with specific primary antibodies against TRX (monoclonal anti-rabbit, 1:50,000; Abcam, Cambridge, MA, USA), TBP-2 (polyclonal anti-rabbit, 0.3 μg/mL, GeneTex, Irvine, CA, USA), and GAPDH (monoclonal anti-mouse, 0.1 μg/mL, Millipore) overnight at 4 °C.

Techniques: Expressing, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Changes in the Expression of TBP-2 in Response to Histone Deacetylase Inhibitor Treatment in Human Endometrial Cells

doi: 10.3390/ijms22031427

Figure Lengend Snippet: Endometrial cell proliferation and TRX, TBP-2 expression after SAHA treatment in stress-induced cells. Oxidative stress was applied using rHMGB-1 10 ng/mL for 24 h. ( A ) Endometrial cell proliferation was measured after 48 h of treatment with 2.5 μM SAHA, which revealed slight increases in three types of cells. ( B ) The proportion of apoptotic cells was measured using flow cytometry. Apoptosis was decreased after treating with rHMGB-1, but was soon overwhelmed with SAHA treatment. ( C ) The relative mRNA expression levels of TBP-2 showed significant reductions after rHMGB-1 treatment compared to the controls. SAHA increased TBP-2 mRNA expression significantly. ( D ) The TRX/TBP-2 ratio was increased in ectopic HESCs after rHMGB-1 treatment only, and then significantly decreased after SAHA treatment. *, p < 0.05 compared to the control; #, p < 0.05 compared to the rHMGB-1-treated cells.

Article Snippet: After blocking with BSA at room temperature for 1 h, the membranes were incubated with specific primary antibodies against TRX (monoclonal anti-rabbit, 1:50,000; Abcam, Cambridge, MA, USA), TBP-2 (polyclonal anti-rabbit, 0.3 μg/mL, GeneTex, Irvine, CA, USA), and GAPDH (monoclonal anti-mouse, 0.1 μg/mL, Millipore) overnight at 4 °C.

Techniques: Expressing, Flow Cytometry

Journal: International Journal of Molecular Sciences

Article Title: Changes in the Expression of TBP-2 in Response to Histone Deacetylase Inhibitor Treatment in Human Endometrial Cells

doi: 10.3390/ijms22031427

Figure Lengend Snippet: Changes after oxidative stress in siTRX transfected endometrial cells. The cells were treated with 10 mg/mL of rHMGB-1 for 24 h, followed by 2.5 μM SAHA treatment for 48 h. ( A ) The mRNA expression of TBP-2 was modified after siTRX transfection. ( B ) The TRX/TBP-2 ratio of mRNA expression revealed a significant decrease in siTRX-transfected cells. ( C ) Western blot was concordant with RT-PCR result. ( D ) Apoptosis was significantly increased in siTRX -transfected cell treated with SAHA, compared to non-SAHA-treated cells (control), and siNC (negative control) cells, respectively. *, p < 0.05 compared to the control; #, p < 0.05 compared to siNC.

Article Snippet: After blocking with BSA at room temperature for 1 h, the membranes were incubated with specific primary antibodies against TRX (monoclonal anti-rabbit, 1:50,000; Abcam, Cambridge, MA, USA), TBP-2 (polyclonal anti-rabbit, 0.3 μg/mL, GeneTex, Irvine, CA, USA), and GAPDH (monoclonal anti-mouse, 0.1 μg/mL, Millipore) overnight at 4 °C.

Techniques: Transfection, Expressing, Modification, Western Blot, Reverse Transcription Polymerase Chain Reaction, Negative Control

Journal:

Article Title: Epidermal Growth Factor Enhances Cellular TATA Binding Protein Levels and Induces RNA Polymerase I- and III-Dependent Gene Activity

doi: 10.1128/MCB.24.12.5119-5129.2004

Figure Lengend Snippet: Analysis of sequences required for EGF induction of the human TBP promoter. JB6 cells were transiently transfected with one of the following hTBP promoter constructs: p−4500/+66, p−736/+66, p−736/+66 etsmut, p−84/+66, p−84/−1, and p−84/−1 etsmut as indicated. Cells in 0.1% FBS-MEM were treated with EGF (12.5 ng/ml) overnight, lysates were prepared, and the resultant luciferase activities were measured as described in Materials and Methods. The values shown are the means ± standard errors of the means of at least four independent experiments.

Article Snippet: Membranes were probed with either rabbit polyclonal anti-human TBP antibody (Upstate Biotechnology), phospho-ERK, phospho-p38 (Cell Signaling), phospho-JNK (Santa Cruz), or mouse monoclonal anti-β-actin (Boehringer Mannheim), as indicated.

Techniques: Transfection, Construct, Luciferase